Difference between revisions of "Part:BBa K3182004"
 
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<partinfo>BBa_K3182004 short</partinfo>
 
<partinfo>BBa_K3182004 short</partinfo>
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Staphylococcus phage endolysin, designed to target Staphylococcus aureus. This part was designed to be assembled with BBa_K3182010.
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For information and characterization of this part please see: <partinfo>BBa_K3182104</partinfo>.
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<br><br>
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Bacteriophage endolysins digest the peptidoglycan wall of bacteria. The LysK is an endolysin targeting gram + bacteria. It has two catalytical domains known as CHAP (cysteine-and histidine depentent amidohydrolase/peptidase) and a central amidase-2 domain together with a cell binding domain. The CHAP domain which is the truncated part expressed in this year’s LiU iGEM (more specifically CHAP C1-C162) team still has lytic activity against staphylococcus. The C1 to C162 amino acids are required for full endopeptidase activity.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 06:10, 17 October 2019


CHAP(K)

For information and characterization of this part please see: BBa_K3182104.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]