Difference between revisions of "Part:BBa K3030015"

Latest revision as of 20:16, 21 October 2019

theophylline riboswitch with optimized RBS

This is a theophylline-responsive riboswitch containing optimized RBS. The sequence is initially designed by Wieland, M. and Hartig, J. S. (2008) with Shines-Dalgarno (SD) sequence. We optimized the RBS by replacing SD sequence with calculated RBS by thermodynamic modeling. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Reference

Wieland, M., Hartig, J.S. (2008). ‘Improved aptazyme design and in vivo screening enable riboswitching in bacteria.’ Angewandte Chemie, 47(14), pp2604-2607. [Online]. Available from: https://www.ncbi.nlm.nih.gov/pubmed/18270990

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 <partinfo>BBa_K3030015 short</partinfo>
-This is an riboswitch responsive to theophylline.
+This is a theophylline-responsive riboswitch containing optimized RBS. The sequence is initially designed by Wieland, M. and Hartig, J. S. (2008) with Shines-Dalgarno (SD) sequence. We optimized the RBS by replacing SD sequence with calculated RBS by thermodynamic modeling.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
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 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3030015 SequenceAndFeatures</partinfo>
+<p></p>
+<h3>Reference</h3>
+Wieland, M., Hartig, J.S. (2008). ‘Improved aptazyme design and in vivo screening enable riboswitching in bacteria.’ Angewandte Chemie, 47(14), pp2604-2607. [Online]. Available from: https://www.ncbi.nlm.nih.gov/pubmed/18270990