Difference between revisions of "Part:BBa K2923003"

 
 
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<partinfo>BBa_K2923003 short</partinfo>
 
<partinfo>BBa_K2923003 short</partinfo>
  
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an aptazyme is an aptamer-ribozyme combinations with intrinsic RNA cleavage activity. this aptazyme is specific for the guanine (liguant).  
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An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme is specific for the guanine or guanosine ligand. It is composed of 3 different parts: a ribozyme module, a communication module and an aptamer module.
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He is composed in 3 differents part, a twister ribozyme module, a communication module and an aptamer module.
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This aptazyme is a ON-switches aptazyme, this mean that in the absence of liguant the RNA does not self-cleave and in the presence of liguant the RNA self-cleaves. This mean that you can sense the guanine in a medium with this aptazyme.
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[[Image: Gua active et inactive.jpg|300px|thumb|center|Figure 1: A ribozyme module is in black. The Shine-Dalgarno (SD) is in blue. The guanine-sensing xpt aptamer (light grey) is connected to the ribozyme via a communication module (in green). The figure is reproduced from [Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.]
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This aptazyme is an ON-switches type of aptazyme.  Its catalytic activity (self-cleavage) requires the presence of ligand.
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[[Image: Aptazyme process.jpg|500px|thumb|center| Figure 2: Schematic graphic of Aptazyme switch-on catalytic activity]]
  
 
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Latest revision as of 17:00, 20 October 2019


Guanine actif aptazyme

An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme is specific for the guanine or guanosine ligand. It is composed of 3 different parts: a ribozyme module, a communication module and an aptamer module.


Figure 1: A ribozyme module is in black. The Shine-Dalgarno (SD) is in blue. The guanine-sensing xpt aptamer (light grey) is connected to the ribozyme via a communication module (in green). The figure is reproduced from [Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.]

This aptazyme is an ON-switches type of aptazyme. Its catalytic activity (self-cleavage) requires the presence of ligand.

Figure 2: Schematic graphic of Aptazyme switch-on catalytic activity

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]