Difference between revisions of "Part:BBa K3168004:Design"

 
 
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===Design Notes===
 
===Design Notes===
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dCas9 and LargeBitNanoLuc are connected by (ggs)5 linker. There is a strep tag at the end for protein purification.
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dCas9 and LargeBitNanoLuc are connected by (GGS)<sub>5</sub> linker. There is a strep tag at the end for protein purification.
  
  
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===Source===
 
===Source===
  
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??
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Basic part [https://parts.igem.org/wiki/index.php?title=Part:BBa_K3168000 (BBa_K3168000)] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K3168002 (BBa_K3168002)].
  
 
===References===
 
===References===
 +
Dixon, A. S., Schwinn, M. K., Hall, M. P., Zimmerman, K., Otto, P., Lubben, T. H., ... & Wood, M. G. (2015). NanoLuc complementation reporter optimized for accurate measurement of protein interactions in cells. ACS chemical biology, 11(2), 400-408.
 +
 +
Zhang, Y., Qian, L., Wei, W., Wang, Y., Wang, B., Lin, P., ... & Cheng, S. (2016). Paired design of dCas9 as a systematic platform for the detection of featured nucleic acid sequences in pathogenic strains. ACS synthetic biology, 6(2), 211-216.

Latest revision as of 13:08, 17 September 2019


dCas9-LargeBitNanoLuc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3378
    Illegal BamHI site found at 4630
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

dCas9 and LargeBitNanoLuc are connected by (GGS)5 linker. There is a strep tag at the end for protein purification.


Source

Basic part (BBa_K3168000) and (BBa_K3168002).

References

Dixon, A. S., Schwinn, M. K., Hall, M. P., Zimmerman, K., Otto, P., Lubben, T. H., ... & Wood, M. G. (2015). NanoLuc complementation reporter optimized for accurate measurement of protein interactions in cells. ACS chemical biology, 11(2), 400-408.

Zhang, Y., Qian, L., Wei, W., Wang, Y., Wang, B., Lin, P., ... & Cheng, S. (2016). Paired design of dCas9 as a systematic platform for the detection of featured nucleic acid sequences in pathogenic strains. ACS synthetic biology, 6(2), 211-216.