Difference between revisions of "Part:BBa K3168003:Design"

Latest revision as of 12:53, 17 September 2019

SmallBitNanoLuc

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 85
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

A flexible (GGS)₅ linker is located in front of the small bit to enable the formation of fusion proteins. A strep tag is also included at the end for protein purification.

Source

Split variant of deep-sea shrimp derived luciferase. Sequence was derived from (Dixon, 2016).

References

Dixon, A. S., Schwinn, M. K., Hall, M. P., Zimmerman, K., Otto, P., Lubben, T. H., ... & Wood, M. G. (2015). NanoLuc complementation reporter optimized for accurate measurement of protein interactions in cells. ACS chemical biology, 11(2), 400-408.

Ohmuro-Matsuyama, Y., & Ueda, H. (2019). Protein-Protein Interaction Assays Using Split-NanoLuc. In Bioluminescence. IntechOpen.

@@ Line 7: / Line 7: @@
 ===Design Notes===
-A flexible (GGS)5 linker is located in front of the small bit to enable the formation of fusion proteins. A strep tag is also included at the end for protein purification.
+A flexible (GGS)<sub>5</sub> linker is located in front of the small bit to enable the formation of fusion proteins. A strep tag is also included at the end for protein purification.
@@ Line 13: / Line 13: @@
 ===Source===
-deep-sea shrimp derived luciferase
+Split variant of deep-sea shrimp derived luciferase. Sequence was derived from (Dixon, 2016).
 ===References===
+Dixon, A. S., Schwinn, M. K., Hall, M. P., Zimmerman, K., Otto, P., Lubben, T. H., ... & Wood, M. G. (2015). NanoLuc complementation reporter optimized for accurate measurement of protein interactions in cells. ACS chemical biology, 11(2), 400-408.
+Ohmuro-Matsuyama, Y., & Ueda, H. (2019). Protein-Protein Interaction Assays Using Split-NanoLuc. In Bioluminescence. IntechOpen.