Difference between revisions of "Part:BBa K3168000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Designed for bacterial expression | + | Designed for bacterial expression. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | S. pyogenes | + | Mutated variant of S. pyogenes derived Cas9, which was a gift from David Liu (Addgene plasmid #62935). |
===References=== | ===References=== | ||
Latest revision as of 12:45, 17 September 2019
dCas9
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1099
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3378
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Designed for bacterial expression.
Source
Mutated variant of S. pyogenes derived Cas9, which was a gift from David Liu (Addgene plasmid #62935).
References
Park, J. J., Dempewolf, E., Zhang, W., & Wang, Z. Y. (2017). RNA-guided transcriptional activation via CRISPR/dCas9 mimics overexpression phenotypes in Arabidopsis. PloS one, 12(6), e0179410.
Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013). Genome engineering using the CRISPR-Cas9 system. Nature protocols, 8(11), 2281.