Difference between revisions of "Part:BBa K3168000:Design"

 
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===Design Notes===
 
===Design Notes===
−
Designed for bacterial expression
+
Designed for bacterial expression.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
  
−
S. pyogenes
+
Mutated variant of S. pyogenes derived Cas9, which was a gift from David Liu (Addgene plasmid #62935).
  
 
===References===
 
===References===
 +
 +
Park, J. J., Dempewolf, E., Zhang, W., & Wang, Z. Y. (2017). RNA-guided transcriptional activation via CRISPR/dCas9 mimics overexpression phenotypes in Arabidopsis. PloS one, 12(6), e0179410.
 +
 +
Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013). Genome engineering using the CRISPR-Cas9 system. Nature protocols, 8(11), 2281.

Latest revision as of 12:45, 17 September 2019


dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3378
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed for bacterial expression.

Source

Mutated variant of S. pyogenes derived Cas9, which was a gift from David Liu (Addgene plasmid #62935).

References

Park, J. J., Dempewolf, E., Zhang, W., & Wang, Z. Y. (2017). RNA-guided transcriptional activation via CRISPR/dCas9 mimics overexpression phenotypes in Arabidopsis. PloS one, 12(6), e0179410.

Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013). Genome engineering using the CRISPR-Cas9 system. Nature protocols, 8(11), 2281.