Difference between revisions of "Part:BBa K115017"

 
(→‎Characterization)
 
(10 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K115017 short</partinfo>
 
<partinfo>BBa_K115017 short</partinfo>
  
−
An RNA thermometer that theoretically switches on translation at 32 degrees Celcius and switched of translation below that temperature. Still to be tested.
+
An [http://2008.igem.org/Team:TUDelft/Temperature_analysis#RNA_thermometer RNA thermometer] which can be used for temperature sensitive post-transcriptional regulation, and which is designed to initiate translation around 32°C.  
 +
 
 +
The image shows the secondary structure of the part after ligation to a protein coding part, as predicted by [http://rna.tbi.univie.ac.at/cgi-bin/RNAfold.cgi RNAfold]. Notice that the 3' end, including the scar and the start codon, does not belong to the part. The blue region is the Shine Dalgarno sequence which is the ribosome binding site.
 +
 
 +
[[Image:BBa_K115017_ss.png|300px]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 17: Line 20:
 
<partinfo>BBa_K115017 parameters</partinfo>
 
<partinfo>BBa_K115017 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
<!--Rice iGEM 2019 improvement -->
 +
===Characterization===
 +
<html>
 +
<p>To characterize this RNA thermometer in vivo, we made an RNA thermometer testing circuit, transformed it into <i>E. coli</i>, and tested the fluorescent output at different temperatures. These graphs show the relative fluorescent intensity of each thermometer between either 25 °C and 30 °C or 25 °C and 37 °C</p>
 +
<img src="https://2019.igem.org/wiki/images/a/a2/T--Rice--RNATcassette.svg" width = 60%>
 +
<p><b>Figure 1.</b>RNA thermometer testing circuit</p>
 +
 +
<img src="https://2019.igem.org/wiki/images/1/16/T--Rice--BBa115017.png" width = 60%>
 +
<p><b>Figure 2.</b> Fluorescence intensity at 25 °C, 30 °C, and 37 °C.</p>
 +
</html>

Latest revision as of 00:30, 22 October 2019

RNA thermometer (ROSE 32C)

An [http://2008.igem.org/Team:TUDelft/Temperature_analysis#RNA_thermometer RNA thermometer] which can be used for temperature sensitive post-transcriptional regulation, and which is designed to initiate translation around 32°C.

The image shows the secondary structure of the part after ligation to a protein coding part, as predicted by [http://rna.tbi.univie.ac.at/cgi-bin/RNAfold.cgi RNAfold]. Notice that the 3' end, including the scar and the start codon, does not belong to the part. The blue region is the Shine Dalgarno sequence which is the ribosome binding site.

BBa K115017 ss.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

To characterize this RNA thermometer in vivo, we made an RNA thermometer testing circuit, transformed it into E. coli, and tested the fluorescent output at different temperatures. These graphs show the relative fluorescent intensity of each thermometer between either 25 °C and 30 °C or 25 °C and 37 °C

Figure 1.RNA thermometer testing circuit

Figure 2. Fluorescence intensity at 25 °C, 30 °C, and 37 °C.