Difference between revisions of "Part:BBa K3058001"
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This part encode a hydroxyisourate hydrolase (from Cyberlindnera jadinii) under the control of the constitutive promoter J23100 and strong RBS B0034. The hydroxyisourate hydrolase encoded can covert hydroxyisourate to 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate. Uricase degrade uric acid into hydroxyisourate. Hydroxyisourate hydrolase can then further degrade the hydroxyisourate. In our project, we express uricase and hydroxyisourate hydrolase in E. coli. It is hoped that the E.coli can lower the uric acid concentration in human alimentary canal, so as to prevent gout disease. | This part encode a hydroxyisourate hydrolase (from Cyberlindnera jadinii) under the control of the constitutive promoter J23100 and strong RBS B0034. The hydroxyisourate hydrolase encoded can covert hydroxyisourate to 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate. Uricase degrade uric acid into hydroxyisourate. Hydroxyisourate hydrolase can then further degrade the hydroxyisourate. In our project, we express uricase and hydroxyisourate hydrolase in E. coli. It is hoped that the E.coli can lower the uric acid concentration in human alimentary canal, so as to prevent gout disease. | ||
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| + | https://2019.igem.org/wiki/images/9/94/T--Hong_Kong_LFC_PC--hiudna.jpg | ||
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| + | https://2019.igem.org/wiki/images/a/aa/T--Hong_Kong_LFC_PC--hiusds.jpg | ||
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| + | https://2019.igem.org/wiki/images/1/18/T--Hong_Kong_LFC_PC--hiuTP.jpg | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 12:27, 20 October 2019
Hydroxyisourate hydrolase from Cyberlindnera jadinii
This part encode a hydroxyisourate hydrolase (from Cyberlindnera jadinii) under the control of the constitutive promoter J23100 and strong RBS B0034. The hydroxyisourate hydrolase encoded can covert hydroxyisourate to 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate. Uricase degrade uric acid into hydroxyisourate. Hydroxyisourate hydrolase can then further degrade the hydroxyisourate. In our project, we express uricase and hydroxyisourate hydrolase in E. coli. It is hoped that the E.coli can lower the uric acid concentration in human alimentary canal, so as to prevent gout disease.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]