Difference between revisions of "Part:BBa K3058000"

Latest revision as of 12:15, 20 October 2019

Uricase from Cyberlindnera jadinii

This part encode a uricase (from Cyberlindnera jadinii) under the control of the constitutive promoter J23100 and strong RBS B0034. The uricase encoded can covert uric acid into hydroxyisourate. In our project, we express it in E. coli. It is hoped that the uricase-expressing E.coli can lower the uric acid concentration in human alimentary canal, so as to prevent gout disease.

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Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 510
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 532
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
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 This part encode a uricase (from Cyberlindnera jadinii) under the control of the constitutive promoter J23100 and strong RBS B0034. The uricase encoded can covert uric acid into hydroxyisourate. In our project, we express it in E. coli. It is hoped that the uricase-expressing E.coli can lower the uric acid concentration in human alimentary canal, so as to prevent gout disease.
+<center>"https://2019.igem.org/wiki/images/f/fc/T--Hong_Kong_LFC_PC--uridna.jpg" </center>
+https://2019.igem.org/wiki/images/0/04/T--Hong_Kong_LFC_PC--urisds.jpg
+https://static.igem.org/mediawiki/parts/5/55/T--Hong_Kong_LFC_PC--Uricasecolor2.jpg
+https://static.igem.org/mediawiki/parts/9/9b/T--Hong_Kong_LFC_PC--Uricasegraph.jpg
+https://2019.igem.org/wiki/images/6/67/T--Hong_Kong_LFC_PC--UricaseTP.jpg
 <!-- Add more about the biology of this part here