Difference between revisions of "Part:BBa K3257011"
 
(4 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3257011 short</partinfo>
 
<partinfo>BBa_K3257011 short</partinfo>
Line 6: Line 5:
  
  
−
<!-- Add more about the biology of this part here
+
====Biology====
−
===Usage and Biology===
+
We measured the relative fluorescence intensity of EGFP expressed by T7 promoter (BBa_K3257000) and this RBS to prove that this RBS can work.
 +
[[File:Fluorescence of EGFP.jpeg|center|400px|thumb|'''Figure 1. The relative fluorescence intensity of EGFP with T7 promoter measured by 96-well plate reader''' EGFP represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding EGFP protein. The Negative represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated EGFP protein. Significant difference between them shows that EGFP can be expressed and function well inside E.coli cells.]]
 +
 
  
 
<!-- -->
 
<!-- -->
Line 18: Line 19:
 
<partinfo>BBa_K3257011 parameters</partinfo>
 
<partinfo>BBa_K3257011 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
====Used by 2021 Fudan====
 +
Please check our parts at https://2021.igem.org/Team:Fudan/Parts

Latest revision as of 02:58, 21 October 2021

T7 RBS

Efficient ribosome binding site from bacteriophage T7 gene 10.


Biology

We measured the relative fluorescence intensity of EGFP expressed by T7 promoter (BBa_K3257000) and this RBS to prove that this RBS can work.

Figure 1. The relative fluorescence intensity of EGFP with T7 promoter measured by 96-well plate reader EGFP represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding EGFP protein. The Negative represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated EGFP protein. Significant difference between them shows that EGFP can be expressed and function well inside E.coli cells.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Used by 2021 Fudan

Please check our parts at https://2021.igem.org/Team:Fudan/Parts