Difference between revisions of "Part:BBa K118011"

 
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This is the promoter for the ''Eschaerichia coli'' JM109 ''cstA'' gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (''cstA'' encodes the carbon starvation protein.)
 
This is the promoter for the ''Eschaerichia coli'' JM109 ''cstA'' gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (''cstA'' encodes the carbon starvation protein.)
  
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===Usage and Biology===
 
===Usage and Biology===
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=ShanghaiTech_China 2022's Characterization=
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In our team's project, giving E. coli the ability to detect and report carbon source starvation helped increase the robustness of the system. Therefore, we chose the PcstA promoter and characterized it fluorescently in DH5α bacteria. After experiments and later sequence improvement, the PcstA promoter can correspond well to carbon source starvation.<br>
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Information about the improved models of our invented parts can be found in<partinfo>BBa_K4115004</partinfo><partinfo>BBa_K4115003</partinfo><partinfo>BBa_K4115027</partinfo>
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<div>
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[[File:Results-PcstA&PcsiE with LVA.png|600px|thumb|center|alt=domestication.|Figrure 1.The result of sfGFP (with LVA) expression under the conditions of different carbon source concentrations]]
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K118011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K118011 SequenceAndFeatures</partinfo>

Latest revision as of 07:19, 12 October 2022


PcstA (glucose-repressible promoter)

This is the promoter for the Eschaerichia coli JM109 cstA gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (cstA encodes the carbon starvation protein.)


Usage and Biology

ShanghaiTech_China 2022's Characterization

In our team's project, giving E. coli the ability to detect and report carbon source starvation helped increase the robustness of the system. Therefore, we chose the PcstA promoter and characterized it fluorescently in DH5α bacteria. After experiments and later sequence improvement, the PcstA promoter can correspond well to carbon source starvation.
Information about the improved models of our invented parts can be found inBBa_K4115004BBa_K4115003BBa_K4115027

domestication.
Figrure 1.The result of sfGFP (with LVA) expression under the conditions of different carbon source concentrations

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]