Difference between revisions of "Part:BBa K2963004"

 
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<partinfo>BBa_K2963004 short</partinfo>
 
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This part contains the racE gene for glutamate racemase from Bacillus. This enzyme mainly catelyse the reation of L-Glumate to D-Glumate.In our project, we express this enzyme simultaneously coexpressing PgsBCA complex in order to try to make poly-gamma-glutamic acid whose D-glutamate content is different.
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This part contains the <i>racE</i> gene encoding racemase from <i>Bacillus sp.</i>. This enzyme mainly catelyses the reation of L-glutamate to D-glutamate. In our project, we coexpress the <i>racE</i> with <i>capB*CA</i> complex in order to produce poly-γ-glutamic acid with different ratio of L-glutamic acid.
  
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===Usage and Biology===
 
===Usage and Biology===
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The <i>racE</i> gene is derived from <i>Bacillus subtilis</i> and it encodes a racemase which can converts L-glutamate to D-glutamate.
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===Characterization===
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We used enzyme activity data to prove the usability of this part. We measured the enzymatic activity under different tac promoters.
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[[image:Figure2-gaohaixin.png|400px]]
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We tested the enzyme activity of the racemase in cells. After induction by IPTG, the results showed that the activity of racemase could be detected under the control of different tac promoters, indicating that this part could be used.
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===References===
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1.Kimura, and K. "Roles and regulation of the glutamate racemase isogenes, racE and yrpC, in Bacillus subtilis." Microbiology 150.9(2004):2911-2920.
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2.Ashiuchi, Makoto , et al. "Differences in effects on DNA gyrase activity between two glutamate racemases of Bacillus subtilis, the poly-γ-glutamate synthesis-linking Glr enzyme and the YrpC (MurI) isozyme." 223.2(2003):221-225.
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Latest revision as of 11:45, 20 October 2019


racE - a glutamate racemase from Bacillus sp.

This part contains the racE gene encoding racemase from Bacillus sp.. This enzyme mainly catelyses the reation of L-glutamate to D-glutamate. In our project, we coexpress the racE with capB*CA complex in order to produce poly-γ-glutamic acid with different ratio of L-glutamic acid.

Usage and Biology

The racE gene is derived from Bacillus subtilis and it encodes a racemase which can converts L-glutamate to D-glutamate.

Characterization

We used enzyme activity data to prove the usability of this part. We measured the enzymatic activity under different tac promoters.

Figure2-gaohaixin.png

We tested the enzyme activity of the racemase in cells. After induction by IPTG, the results showed that the activity of racemase could be detected under the control of different tac promoters, indicating that this part could be used.

References

1.Kimura, and K. "Roles and regulation of the glutamate racemase isogenes, racE and yrpC, in Bacillus subtilis." Microbiology 150.9(2004):2911-2920.

2.Ashiuchi, Makoto , et al. "Differences in effects on DNA gyrase activity between two glutamate racemases of Bacillus subtilis, the poly-γ-glutamate synthesis-linking Glr enzyme and the YrpC (MurI) isozyme." 223.2(2003):221-225.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]