Difference between revisions of "Part:BBa K3257045:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We mutated the 60th nucleotide from C to A, the 61st nucleotide from A to T, 136th nucleotide from C to T, the 490th nucleotide from T to A, the 500th nucleotide from A to G and the 1001st nucleotide from C to G of | + | We cut out BBa_C0012 between the 9th and 1153rd nucleotide, mutated the 60th nucleotide from C to A, the 61st nucleotide from A to T, 136th nucleotide from C to T, the 490th nucleotide from T to A, the 500th nucleotide from A to G and the 1001st nucleotide from C to G of it and added a stop codon at the end of it. |
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===Source=== | ===Source=== | ||
Latest revision as of 17:12, 6 September 2019
Improved LacI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We cut out BBa_C0012 between the 9th and 1153rd nucleotide, mutated the 60th nucleotide from C to A, the 61st nucleotide from A to T, 136th nucleotide from C to T, the 490th nucleotide from T to A, the 500th nucleotide from A to G and the 1001st nucleotide from C to G of it and added a stop codon at the end of it.
Source
Mutated from BBa_C0012
References
AJ Meyer et al. Escherichia coli "Marionette" strains with 12 highly optimized small-molecule sensors. Nat Chem Biol. 2019 Feb;15(2):196-204. doi: 10.1038/s41589-018-0168-3. Epub 2018 Nov 26.