Difference between revisions of "Part:BBa K2976007"
 
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===Usage and Biology===
 
===Usage and Biology===
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<p>Lysosomal associated membrane protein 2 (Lamp 2) is a member of a family of membrane glycoproteins, which is highly expressed on the surface of exosomes. Much research has indicated that peptides fused to the N terminus of Lamp2 could be effectively displayed on the outer surface of exosomes. In 2019 CPU_CHINA project, we display PD-L1 targeting peptide on outer surface of exosomes and adopt this targeting exosome system to deliver hsa-let-7f into Mtb-infected macrophages specifically.
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<p>Lysosomal associated membrane protein 2 (Lamp 2) is a member of a family of membrane glycoproteins, which is highly expressed on the surface of exosomes. Much research has indicated that peptides fused to the N terminus of Lamp2 could be effectively displayed on the outer surface of exosomes. In 2019 CPU_CHINA project, we display PD-L1 targeting peptide on outer surface of exosomes and adopt this targeting exosome system to deliver hsa-let-7f into <i>Mtb</i>-infected macrophages specifically.
 
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===Characteristics===
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<p>According to the design of our plasmid, the sequence of lamp2 and HA tag was combined for expressing fusion protein, since the HA tag was characterized by western blot(Fig.1), the protein lamp2 was also proved to be expressed together with HA tag.
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[[File:T--CPUCHINA--HAtag.jpg|300px|thumb|center|Figure 1: Western blot result of exosome protein.]]
  
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<p>For more details, please check out our [https://2019.igem.org/Team:CPU_CHINA/Demonstrate'''demonstrate page'''].</p>
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===Usage and Biology===
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Latest revision as of 21:45, 21 October 2019

Lamp 2

Usage and Biology

Lysosomal associated membrane protein 2 (Lamp 2) is a member of a family of membrane glycoproteins, which is highly expressed on the surface of exosomes. Much research has indicated that peptides fused to the N terminus of Lamp2 could be effectively displayed on the outer surface of exosomes. In 2019 CPU_CHINA project, we display PD-L1 targeting peptide on outer surface of exosomes and adopt this targeting exosome system to deliver hsa-let-7f into Mtb-infected macrophages specifically.

Characteristics

According to the design of our plasmid, the sequence of lamp2 and HA tag was combined for expressing fusion protein, since the HA tag was characterized by western blot(Fig.1), the protein lamp2 was also proved to be expressed together with HA tag.

Figure 1: Western blot result of exosome protein.

For more details, please check out our demonstrate page.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]