Difference between revisions of "Part:BBa K2996002:Design"

 
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===Design Notes===
 
===Design Notes===
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Since
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The structure of RSRL sequence is complicated and it is relatively short, we suggest direct synthesis when applying this biobrick.
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we intend to have constitutive expression of this part, so promotor J23117 and terminator
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Also, be careful with the number of necleotides you are adding, since this device only works when the reading frame can be precisely regulated.
  
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===Source===
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RSRL cassette contains 69 bp of the leader2.1 and the adjacent CRISPR-spacer-CRISPR amplified from E. coli K12 str. MG.1655.
  
  
  
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===Source===
 
  
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cluc is cloned from
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===References===
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zinc finger is cloned from
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1.Amlinger L , Hoekzema M , Wagner E G H , et al. Fluorescent CRISPR Adaptation Reporter for rapid quantification of spacer acquisition[J]. Scientific Reports, 2017, 7(1):10392.
  
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2.Díez-Villase?or, César, Guzmán, Noemí M, Almendros, Cristóbal, et al. CRISPR-spacer integration reporter plasmids reveal distinct genuine acquisition specificities among CRISPR-Cas I-E variants of Escherichia coli, [J]. RNA Biology, 2013, 10(5):792-802.
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===References===
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Latest revision as of 00:48, 11 October 2019


minimal CRISPR array-A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The structure of RSRL sequence is complicated and it is relatively short, we suggest direct synthesis when applying this biobrick. Also, be careful with the number of necleotides you are adding, since this device only works when the reading frame can be precisely regulated.

Source

RSRL cassette contains 69 bp of the leader2.1 and the adjacent CRISPR-spacer-CRISPR amplified from E. coli K12 str. MG.1655.



References

1.Amlinger L , Hoekzema M , Wagner E G H , et al. Fluorescent CRISPR Adaptation Reporter for rapid quantification of spacer acquisition[J]. Scientific Reports, 2017, 7(1):10392.

2.Díez-Villase?or, César, Guzmán, Noemí M, Almendros, Cristóbal, et al. CRISPR-spacer integration reporter plasmids reveal distinct genuine acquisition specificities among CRISPR-Cas I-E variants of Escherichia coli, [J]. RNA Biology, 2013, 10(5):792-802.