Difference between revisions of "Part:BBa K2969010"

 
 
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<partinfo>BBa_K2969010 short</partinfo>
 
<partinfo>BBa_K2969010 short</partinfo>
  
An engineered CI transcription factor in which the TVMV recognition and cut site is added.
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An engineered CI transcription factor. We insert the TVMV protease recognition sequence between N-terminal and C-terminal of CI. Because the structure of CI has been characterized, the function of the fragment between N-terminal and C-terminal is linking. So, we choose this inserting site to avoid bigger influence. In our project, this kind of CI can be cleaved by TVMV so that it will lose activity.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 17:47, 19 October 2019


CI-TVMVsite

An engineered CI transcription factor. We insert the TVMV protease recognition sequence between N-terminal and C-terminal of CI. Because the structure of CI has been characterized, the function of the fragment between N-terminal and C-terminal is linking. So, we choose this inserting site to avoid bigger influence. In our project, this kind of CI can be cleaved by TVMV so that it will lose activity.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]