Difference between revisions of "Part:BBa K112100:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K112100 short</partinfo>
 
<partinfo>BBa_K112100 short</partinfo>
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===Design Notes===
 
===Design Notes===
 
This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.
 
This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.
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PCR mv001/mv002 on pBca9145-Bca1010    (1060bp, EcoRI/BamHI/DpnI)
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<pre>
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PCR mv001/mv002 on pBca9145-Bca1010    (1060bp, EcoRI/BamHI)
 
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
Product is pBca1256-K112100
 
Product is pBca1256-K112100
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mv002 Reverse Biobricking of <Cre!
 
mv002 Reverse Biobricking of <Cre!
 
cgttaGGATCCttaatcgccatcttccagc
 
cgttaGGATCCttaatcgccatcttccagc
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</pre>
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===Source===
 
===Source===
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+
<pre>
 
PCR mv001/mv002 on pBca9145-Bca1010    (1060bp, EcoRI/BamHI)
 
PCR mv001/mv002 on pBca9145-Bca1010    (1060bp, EcoRI/BamHI)
 
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
 
Product is pBca1256-K112100
 
Product is pBca1256-K112100
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</pre>
  
 
===References===
 
===References===

Latest revision as of 02:34, 15 August 2008

Cre enzyme without start codon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 39
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. 

PCR mv001/mv002 on pBca9145-Bca1010    (1060bp, EcoRI/BamHI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112100
-----------------------------------------------
mv001	Forward Biobricking of <Cre!
ccataGAATTCatgAGATCTtccaatttactgaccgtacacc
mv002	Reverse Biobricking of <Cre!
cgttaGGATCCttaatcgccatcttccagc

Source

PCR mv001/mv002 on pBca9145-Bca1010    (1060bp, EcoRI/BamHI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112100

References