Difference between revisions of "Part:BBa K2927004"
 
(One intermediate revision by one other user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2927004 short</partinfo>
 
<partinfo>BBa_K2927004 short</partinfo>
  
−
TEV enzyme cut the TEV cutting site to separate the Cas12a protein from the composition (10X His-MBP-TEV-Cas12a) in our project. It makes Cas12a protein be collected in the solution. Furthermore, the sequence of the TEV cutting site is used by E.coli.
+
This sequence encoded the peptide motif that is recognized by Tobaco Etch Virus (TEV) enzyme. TEV motif is used in removed the purification-associated compositions (10XHis and MBP) after Cas12a protein purification.  
  
 
'''Reference'''<br>
 
'''Reference'''<br>

Latest revision as of 04:48, 16 October 2019


Tobaco Etch Virus (TEV) motif

This sequence encoded the peptide motif that is recognized by Tobaco Etch Virus (TEV) enzyme. TEV motif is used in removed the purification-associated compositions (10XHis and MBP) after Cas12a protein purification.

Reference
Janice S. Chen, Enbo Ma, Lucas B. Harrington, Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018) Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]