Difference between revisions of "Part:BBa K2927002"
 
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__NOTOC__
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<partinfo>BBa_K2927002 short</partinfo>
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This sequence encoded 10 connected histidines, which can be used for protein purification through strong interaction with Nickel (II) ion. It belongs to the compositions of Cas12a expression plasmid (10X His-MBP-TEV-Cas12a).
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'''Reference'''<br>
 
'''Reference'''<br>
 
Janice S. Chen, Enbo Ma, Lucas B. Harrington,  Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018)
 
Janice S. Chen, Enbo Ma, Lucas B. Harrington,  Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018)
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>'''Sequence and Features'''</span><br>
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<partinfo>BBa_K2927002 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K2927002 parameters</partinfo>
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<!-- -->

Latest revision as of 04:38, 16 October 2019

10X His

This sequence encoded 10 connected histidines, which can be used for protein purification through strong interaction with Nickel (II) ion. It belongs to the compositions of Cas12a expression plasmid (10X His-MBP-TEV-Cas12a).

Reference
Janice S. Chen, Enbo Ma, Lucas B. Harrington, Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018) Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]