Difference between revisions of "Part:BBa K2909008"
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This part is a reporter construct with a Clover GFP reporter gene tagged with a C-terminal HiBiT tag under the control of a nitrate-inducible Chlamydomonas reinhardtii promoter called NIT1. It also contains a Paromomycin resistance cassette called AphVIII to select the recombinant clones.<br> | This part is a reporter construct with a Clover GFP reporter gene tagged with a C-terminal HiBiT tag under the control of a nitrate-inducible Chlamydomonas reinhardtii promoter called NIT1. It also contains a Paromomycin resistance cassette called AphVIII to select the recombinant clones.<br> | ||
| − | This part has been designed to be used as a reporter construct to characterize our C-terminal HiBiT tag for the Chlamydomonas reinhardtii MoClo Kit(BBa_K2909001).<br> | + | This part has been designed to be used as a reporter construct to characterize our C-terminal HiBiT tag for the Chlamydomonas reinhardtii MoClo Kit (BBa_K2909001).<br> |
| − | This part is a linear DNA that is the result of the digestion of the level M plasmid by the type IIS BsaI | + | This part is a linear DNA that is the result of the digestion of the level M plasmid by the type IIS BsaI restriction enzyme. It is this linear DNA that is integrated into the genomic DNA of Chlamydomonas reinhardtii during the transformation. |
<h3> 2- Usage in iGEM projects </h3> | <h3> 2- Usage in iGEM projects </h3> | ||
| − | Bio | + | Bio(oil)gical Factory (iGEM Sorbonne Université 2019) |
=='''Characterization'''== | =='''Characterization'''== | ||
Latest revision as of 11:25, 4 September 2019
ParoR_CloverGFP-HiBiT
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 5
Illegal EcoRI site found at 1567
Illegal PstI site found at 2806 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 5
Illegal EcoRI site found at 1567
Illegal NheI site found at 269
Illegal PstI site found at 2806
Illegal NotI site found at 1779 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 5
Illegal EcoRI site found at 1567
Illegal BamHI site found at 11
Illegal XhoI site found at 2320 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 5
Illegal EcoRI site found at 1567
Illegal PstI site found at 2806 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 5
Illegal EcoRI site found at 1567
Illegal PstI site found at 2806 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 3466
Introduction
1- Biological background
This part is a reporter construct with a Clover GFP reporter gene tagged with a C-terminal HiBiT tag under the control of a nitrate-inducible Chlamydomonas reinhardtii promoter called NIT1. It also contains a Paromomycin resistance cassette called AphVIII to select the recombinant clones.
This part has been designed to be used as a reporter construct to characterize our C-terminal HiBiT tag for the Chlamydomonas reinhardtii MoClo Kit (BBa_K2909001).
This part is a linear DNA that is the result of the digestion of the level M plasmid by the type IIS BsaI restriction enzyme. It is this linear DNA that is integrated into the genomic DNA of Chlamydomonas reinhardtii during the transformation.
2- Usage in iGEM projects
Bio(oil)gical Factory (iGEM Sorbonne Université 2019)
Characterization
References
- Crozet, P. et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. 7, 2074–2086 (2018).
- 1.Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLoS ONE 6, e16765 (2011).