Difference between revisions of "Part:BBa K2927001"
 
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<partinfo>BBa_K2927001 short</partinfo>
 
<partinfo>BBa_K2927001 short</partinfo>
  
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The part of RBS is the site which recruits ribosome during the initiation of Cas12a protein translation. Furthermore, RBS let ribosome bind on the sequence and start for transcription. The RBS is used by E.coli for transcription.
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Ribosome entry site (RBS) recruits ribosome during the initiation of Cas12a protein translation.  
  
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'''Reference'''<br>
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Janice S. Chen, Enbo Ma, Lucas B. Harrington,  Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018)
 
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>'''Sequence and Features'''</span><br>
 
<span class='h3bb'>'''Sequence and Features'''</span><br>
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Janice S. Chen, Enbo Ma, Lucas B. Harrington,  Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018)
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<partinfo>BBa_K2927001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2927001 SequenceAndFeatures</partinfo>
  
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===Part Contributions===
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'''Group:''' iGEM22_Stony_Brook
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'''Authors:''' Maulik Masaliya
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'''Contribution Summary'''<br>
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This RBS is part of the Protein S gene circuit <partinfo>BBa_K4235030</partinfo>, in the vector <partinfo>BBa_K4235016</partinfo>. We successfully expressed protein S from bacterial expression system as highlighted on our team wiki. 
  
 
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Latest revision as of 01:39, 27 September 2022


Ribosome binding site

Ribosome entry site (RBS) recruits ribosome during the initiation of Cas12a protein translation.

Reference
Janice S. Chen, Enbo Ma, Lucas B. Harrington, Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018) Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Part Contributions

Group: iGEM22_Stony_Brook

Authors: Maulik Masaliya

Contribution Summary
This RBS is part of the Protein S gene circuit BBa_K4235030, in the vector BBa_K4235016. We successfully expressed protein S from bacterial expression system as highlighted on our team wiki.