Difference between revisions of "Part:BBa K2973007:Design"
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===Design Notes=== | ===Design Notes=== | ||
The toehold switch sequence was taken from Pardee et al., 2016 . | The toehold switch sequence was taken from Pardee et al., 2016 . | ||
| − | + | Beta-lactamase needs to be truncated in its N-terminal end in order to work properly. Therefore, we removed the signal peptide from its sequence in order to do our in vitro experiments | |
Latest revision as of 14:33, 21 October 2019
32B Toehold Switch_β-lactamase_no signal peptide
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The toehold switch sequence was taken from Pardee et al., 2016 . Beta-lactamase needs to be truncated in its N-terminal end in order to work properly. Therefore, we removed the signal peptide from its sequence in order to do our in vitro experiments
Source
Pardee, Keith, et al. “Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.” Cell, vol. 165, no. 5, 2016, pp. 1255–1266., doi:10.1016/j.cell.2016.04.059.
References
Pardee, Keith, et al. “Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.” Cell, vol. 165, no. 5, 2016, pp. 1255–1266., doi:10.1016/j.cell.2016.04.059.
Green, Alexander A., et al. “Toehold Switches: De-Novo-Designed Regulators of Gene Expression.” Cell, vol. 159, no. 4, 2014, pp. 925–939., doi:10.1016/j.cell.2014.10.002.
Qureshi, Sohail A. “β-Lactamase: an Ideal Reporter System for Monitoring Gene Expression in Live Eukaryotic Cells.” BioTechniques, vol. 42, no. 1, 2007, pp. 91–96., doi:10.2144/000112292.
Boehle, Katherine E., et al. “Paper-Based Enzyme Competition Assay for Detecting Falsified β-Lactam Antibiotics.” ACS Sensors, vol. 3, no. 7, 2018, pp. 1299–1307., doi:10.1021/acssensors.8b00163.