Difference between revisions of "Part:BBa K137076:Experience"

Latest revision as of 07:18, 14 August 2008

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K137076

Strain: This part will only work in a catalase deficient strain of E. coli. Our testing strain, JI377, is DE(katE, katG, ahp). No H₂O₂ is produced in a catalase proficient strain, such as DH10B.
Culture conditions: Saturated LB culture backdiluted 1:100 into SOC +1mM IPTG. Let shake at 37C until the culture reaches an OD600 = 0.8. Bring culture to 10nM AHL and let shake at 37 C for 3 hrs. Pellet cells and assay supernatant for hydrogen peroxide.
Results: Under these conditions the supernatant had ~50 uM H₂O₂ prior to induction and ~200 uM ~200uM H₂O₂ 3hrs post induction with AHL.

User Reviews

UNIQf14bed568a2a1189-partinfo-00000000-QINU UNIQf14bed568a2a1189-partinfo-00000001-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_K137076===
+*Strain: This part will only work in a catalase deficient strain of E. coli. Our testing strain, JI377, is DE(katE, katG, ahp). No H<sub>2</sub>O<sub>2</sub> is produced in a catalase proficient strain, such as DH10B.
+*Culture conditions: Saturated LB culture backdiluted 1:100 into SOC +1mM IPTG. Let shake at 37C until the culture reaches an OD600 = 0.8. Bring culture to 10nM AHL and let shake at 37 C for 3 hrs. Pellet cells and assay supernatant for hydrogen peroxide.
+*Results: Under these conditions the supernatant had ~50 uM  H<sub>2</sub>O<sub>2</sub> prior to induction and ~200 uM ~200uM H<sub>2</sub>O<sub>2</sub> 3hrs post induction with AHL.
 ===User Reviews===