Difference between revisions of "Part:BBa K2982000:Design"

Latest revision as of 04:37, 7 August 2019

Coding sequence for wild type IsPETase

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 348
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 304
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 348
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 348
Illegal AgeI site found at 627
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Extra amino acids are added to the N-terminus and C-terminus of the codon optimized DNA sequence used for expression of wild type Ideonella sakainesis PETase.

Source

Sequence of wild type PETase optimized for Escherichia coli from 'Characterization and engineering of a plastic-degrading aromatic polyesterase', PNAs, 2018.

References

Austin, H. P., Allen, M. D., Donohoe, B. S., Rorrer, N. A., Kearns, F. L., Silveira, R. L., . . . Beckham, G. T. (2018). Characterization and engineering of a plastic-degrading aromatic polyesterase. Proceedings of the National Academy of Sciences, 115(19). doi:10.1073/pnas.1718804115

@@ Line 13: / Line 13: @@
 ===Source===
-Sequence of wild type PETase optimized for Escherichia coli from Structural Insight into Molecular Mechanism of Poly (ethylene terephthalate) Degradation.
+Sequence of wild type PETase optimized for Escherichia coli from 'Characterization and engineering of a plastic-degrading aromatic polyesterase', PNAs, 2018.
 ===References===
-Joo, S., Cho, I. J., Seo, H., Son, H. F., Sagong, H., Shin, T. J., . . . Kim, K. (2018). Structural insight into molecular mechanism of poly(ethylene terephthalate) degradation. Nature Communications, 9(1). doi:10.1038/s41467-018-02881-1
+Austin, H. P., Allen, M. D., Donohoe, B. S., Rorrer, N. A., Kearns, F. L., Silveira, R. L., . . . Beckham, G. T. (2018). Characterization and engineering of a plastic-degrading aromatic polyesterase. Proceedings of the National Academy of Sciences, 115(19). doi:10.1073/pnas.1718804115