Difference between revisions of "Part:BBa J119438"

 
 
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<partinfo>BBa_J119438 short</partinfo>
 
<partinfo>BBa_J119438 short</partinfo>
  
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The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. These ends allow the part to be inserted into an Eco RI cut site with either strand on top or bottom.   
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The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. The i-motif is shown on the coding strand of this sequence. If the part was to be ligated into a plasmid that had been digested with Eco RI, it could be ligated in either orientation (i-motif on the coding strand or G-quadruplex on the coding strand).  The i-motif/G-quadruplex-forming sequence comes from the human BCL-2 promoter.
  
 
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Latest revision as of 21:18, 18 July 2019


BCL-2 promoter i-Motif and G quadruplex

The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. The i-motif is shown on the coding strand of this sequence. If the part was to be ligated into a plasmid that had been digested with Eco RI, it could be ligated in either orientation (i-motif on the coding strand or G-quadruplex on the coding strand). The i-motif/G-quadruplex-forming sequence comes from the human BCL-2 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]