Difference between revisions of "Part:BBa J100459:Experience"
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| − | [[File: | + | [[File: J100459_GFP.PNG|350px]] |
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| + | [[File:bidirectional_promoter.png|500px|]] | ||
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| + | The graph on the right shows mScarlet fluorescent protein (SFP) levels when the promoter is initiating transcription to the "right." The graph on the left shows GFP output when the promoter is initiating transcription to the "left." The photograph below shows three experimental clones of the promoter J100459. Notice the cultures in the first three tubes look orange because both GFP and SFP are being produced in the cells. You can compare the colors with the SFP (tube 4) and GFP (tube 5) control tubes. | ||
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Latest revision as of 16:59, 12 February 2019
The graph on the right shows mScarlet fluorescent protein (SFP) levels when the promoter is initiating transcription to the "right." The graph on the left shows GFP output when the promoter is initiating transcription to the "left." The photograph below shows three experimental clones of the promoter J100459. Notice the cultures in the first three tubes look orange because both GFP and SFP are being produced in the cells. You can compare the colors with the SFP (tube 4) and GFP (tube 5) control tubes.
After adjusting the fluorescence over absorbance values obtained from the Synergy machine using scarlet fluorescent protein (SFP) wavelengths, it was observed that all three experimental samples showed significant difference compared to the negative control (GFP) and the promoter strength was almost twice as strong as the positive control (SFP).
The fluorescence over absorbance values were also adjusted using GFP wavelengths. GFP served as the positive control, with SFP as the negative control. All three experimental samples showed significant difference compared to the negative control, with experimental 1 showing no significant difference compared to the positive control. The error bars show standard error.
Data from the graphs show that the Staphylococcal enterotoxin B promoter express strong promoter strength for both gene expressions of SFP and GFP. DNA sequencing further confirmed that the promoter is a bidirectional promoter.
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