Difference between revisions of "Part:BBa J119413"
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<partinfo>BBa_J119413 short</partinfo> | <partinfo>BBa_J119413 short</partinfo> | ||
| − | This promoter is pTac with an i-motif forming sequence on the coding strand. The -10 element is included as a loop in the i-motif. The p-Tac -35 and -10 elements were adjusted to have 17 base pairs between them, the optimal spacing, and an i-motif sequence was chosen to fit around the -10 element and in the center of the promoter. | + | This promoter is pTac with an i-motif forming sequence on the coding strand. The i-motif sequence was a modified form of the human c-Myc promoter's Nuclease Hypersensitive Element III. The -10 element is included as a loop in the i-motif. The p-Tac -35 and -10 elements were adjusted to have 17 base pairs between them, the optimal spacing, and an i-motif sequence was chosen to fit around the -10 element and in the center of the promoter. |
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Latest revision as of 16:12, 5 January 2019
G41 - i-motif sequence on pTac coding strand
This promoter is pTac with an i-motif forming sequence on the coding strand. The i-motif sequence was a modified form of the human c-Myc promoter's Nuclease Hypersensitive Element III. The -10 element is included as a loop in the i-motif. The p-Tac -35 and -10 elements were adjusted to have 17 base pairs between them, the optimal spacing, and an i-motif sequence was chosen to fit around the -10 element and in the center of the promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]