Difference between revisions of "Part:BBa M50477"
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Dynamic range experimentation concluded that no meffRed is visibly produced after heat-shock at a variety of warm temperatures after 60 minutes; Western blot analysis also confirmed these results. Future experimentation could look at reverse transcription analysis to see if mRNA is being produced after heat-shock. If not, utilizing a composite part with a different heat-shock promoter is recommended. | Dynamic range experimentation concluded that no meffRed is visibly produced after heat-shock at a variety of warm temperatures after 60 minutes; Western blot analysis also confirmed these results. Future experimentation could look at reverse transcription analysis to see if mRNA is being produced after heat-shock. If not, utilizing a composite part with a different heat-shock promoter is recommended. | ||
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| + | <partinfo>BBa_M50477 short</partinfo> | ||
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| + | <partinfo>BBa_M50477 SequenceAndFeatures</partinfo> | ||
Latest revision as of 01:19, 12 December 2018
Heat-shock-indued chromoprotein expression plasmid (pHeat)
This plasmid senses warm temperatures using the heat-shock protein 70 promoter (hsp70) and produces red pigment (meffRed) when activated. The warmer the temperature, the more red pigment that is produced. meffRed is tagged with a 6X-His tag, which facilitates Western blot analysis of meffRed production.
Dynamic range experimentation concluded that no meffRed is visibly produced after heat-shock at a variety of warm temperatures after 60 minutes; Western blot analysis also confirmed these results. Future experimentation could look at reverse transcription analysis to see if mRNA is being produced after heat-shock. If not, utilizing a composite part with a different heat-shock promoter is recommended.
Heat-shock-indued chromoprotein expression plasmid (pHeat)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 547