Difference between revisions of "Part:BBa M50499"

Latest revision as of 23:53, 11 December 2018

Catabolite Activator Protein (CAP) Repressed Promoter

We placed the constitutive promoter (BBa_S05450, iGEM) immediately upstream from a catabolite activator protein (CAP) binding site (BBa_M36547, iGEM). When CAP binds to the binding site, expression will be repressed.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 1059
Illegal PstI site found at 688
Illegal PstI site found at 849
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 144
Illegal NheI site found at 167
Illegal PstI site found at 688
Illegal PstI site found at 849
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 1059
Illegal PstI site found at 688
Illegal PstI site found at 849
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 1059
Illegal PstI site found at 688
Illegal PstI site found at 849
1000
COMPATIBLE WITH RFC[1000]

Constitutive promoter (BBa_S05450, iGEM) and catabolite activator protein (CAP) binding site (BBa_M36547, iGEM)

@@ Line 3: / Line 3: @@
 <partinfo>BBa_M50499 short</partinfo>
-We placed the constitutive promoter (BBa_S05450, iGEM) immediately upstream from a CAP binding site (BBa_M36547, iGEM) in Plasmid E.
+We placed the constitutive promoter (BBa_S05450, iGEM) immediately upstream from a catabolite activator protein (CAP) binding site (BBa_M36547, iGEM). When CAP binds to the binding site, expression will be repressed.
-For our experiments, we placed this part upstream from GFP in order to assay expression levels through fluorescent intensity.
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 ===Usage and Biology===
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@@ Line 14: / Line 13: @@
 <partinfo>BBa_M50499 SequenceAndFeatures</partinfo>
+Constitutive promoter (BBa_S05450, iGEM) and catabolite activator protein (CAP) binding site (BBa_M36547, iGEM)
 <!-- Uncomment this to enable Functional Parameter display