Difference between revisions of "Part:BBa K2832000:Design"
 
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===Design Notes===
 
===Design Notes===
 
https://static.igem.org/mediawiki/parts/9/9b/PbamE_design_diagram.png
 
https://static.igem.org/mediawiki/parts/9/9b/PbamE_design_diagram.png
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PbamE Design Diagram. The bamE promoter sequence binds Sigma Factor E (RpoE) when freed by the rpoE pathway. The pathway detects membrane damage via misfolded outer membrane proteins. mRFP1 signal indicates successful activation of the pathway.
  
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https://static.igem.org/mediawiki/parts/f/fb/PbamE_plasmid_map.png
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FSU Promoter BBa_K2832000 inside plasmid pSB1C3.
 
===Source===
 
===Source===
  

Latest revision as of 20:50, 1 December 2018


PbamE - Inducible Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 13
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PbamE_design_diagram.png PbamE Design Diagram. The bamE promoter sequence binds Sigma Factor E (RpoE) when freed by the rpoE pathway. The pathway detects membrane damage via misfolded outer membrane proteins. mRFP1 signal indicates successful activation of the pathway.

PbamE_plasmid_map.png FSU Promoter BBa_K2832000 inside plasmid pSB1C3.

Source

E. coli K12 genome.

References