Difference between revisions of "Part:BBa K2832003:Experience"
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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After exposing our different test cells to different frequencies and amplitudes, we found that cells did seem to respond differently to different sonic stress conditions based on the promoters they carried. We fund that cell growth was most aided by 2khz at 100db. On the other hand, cell growth was most disrupted by 8khz and 100db, and we think this frequency may actually be killing cells and thus preventing growth. In general, the PSPA system was the most sound responsive system and K112402 was likely a constitutive promoter
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how you used this part and how it worked out.
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===Applications of BBa_K2832003===
 
===Applications of BBa_K2832003===
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The graphs below include all the promoters tested with PpspA
 
https://static.igem.org/mediawiki/parts/7/76/Ambient_sound_results_graph.png
 
https://static.igem.org/mediawiki/parts/7/76/Ambient_sound_results_graph.png
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Stationary Phase Cells vs the Cells in Ambient sound
 
https://static.igem.org/mediawiki/parts/c/cf/Ambient_sound_results.png
 
https://static.igem.org/mediawiki/parts/c/cf/Ambient_sound_results.png
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Data Table for Stationary Phase Cells vs the Cells in Ambient sound
 
https://static.igem.org/mediawiki/parts/1/16/Ambient_sound_results2.png
 
https://static.igem.org/mediawiki/parts/1/16/Ambient_sound_results2.png
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Stationary Phase Cells vs the Cells in Ambient sound
 
https://static.igem.org/mediawiki/parts/3/35/Ambient_sound_results2_table.png
 
https://static.igem.org/mediawiki/parts/3/35/Ambient_sound_results2_table.png
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Data Table for Stationary Phase Cells vs the Cells in Ambient sound
 
https://static.igem.org/mediawiki/parts/f/ff/8kHz_80_dB_sound.png
 
https://static.igem.org/mediawiki/parts/f/ff/8kHz_80_dB_sound.png
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Stationary Phase Cells vs Cells exposed to 8 kHz, 80dB sound
 
https://static.igem.org/mediawiki/parts/0/04/8kHz_80_dB_data_table.png
 
https://static.igem.org/mediawiki/parts/0/04/8kHz_80_dB_data_table.png
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Data Table For Stationary Phase Cells vs Cells exposed to 8 kHz, 80dB sound
 
https://static.igem.org/mediawiki/parts/d/d0/8_kHz_100_dB_sound.png
 
https://static.igem.org/mediawiki/parts/d/d0/8_kHz_100_dB_sound.png
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Stationary Phase Cells vs Cells exposed to 8 kHz, 100dB sound
 
https://static.igem.org/mediawiki/parts/7/71/8_kHz_100_dB_sound_data_table.png
 
https://static.igem.org/mediawiki/parts/7/71/8_kHz_100_dB_sound_data_table.png
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Data Table for Stationary Phase Cells vs Cells exposed to 8 kHz, 100dB sound
 
https://static.igem.org/mediawiki/parts/a/ac/2kHz_100_dB_sound.png
 
https://static.igem.org/mediawiki/parts/a/ac/2kHz_100_dB_sound.png
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Stationary Phase Cells vs Cells exposed to 2 kHz, 100dB sound
 
https://static.igem.org/mediawiki/parts/1/1a/2_kHz_100_dB_data_table.png
 
https://static.igem.org/mediawiki/parts/1/1a/2_kHz_100_dB_data_table.png
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Data Table for Stationary Phase Cells vs Cells exposed to 2 kHz, 100dB sound
 
https://static.igem.org/mediawiki/parts/3/3e/2_kHz_100_dB_comparative_graph.png
 
https://static.igem.org/mediawiki/parts/3/3e/2_kHz_100_dB_comparative_graph.png
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This Graph describes the expression levels of incubated cells divided by the expression of the cells in stationary phase cells and their response to sound.
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https://static.igem.org/mediawiki/parts/1/1e/2_kHz_100_dB_comparative_graph_data_table.png
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Data table for expression levels of incubated cells divided by the expression of the cells in stationary phase cells and their response to sound.
 
===User Reviews===
 
===User Reviews===
 
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Latest revision as of 20:45, 3 December 2018


After exposing our different test cells to different frequencies and amplitudes, we found that cells did seem to respond differently to different sonic stress conditions based on the promoters they carried. We fund that cell growth was most aided by 2khz at 100db. On the other hand, cell growth was most disrupted by 8khz and 100db, and we think this frequency may actually be killing cells and thus preventing growth. In general, the PSPA system was the most sound responsive system and K112402 was likely a constitutive promoter

Applications of BBa_K2832003

The graphs below include all the promoters tested with PpspA Ambient_sound_results_graph.png Stationary Phase Cells vs the Cells in Ambient sound Ambient_sound_results.png Data Table for Stationary Phase Cells vs the Cells in Ambient sound Ambient_sound_results2.png Stationary Phase Cells vs the Cells in Ambient sound Ambient_sound_results2_table.png Data Table for Stationary Phase Cells vs the Cells in Ambient sound 8kHz_80_dB_sound.png Stationary Phase Cells vs Cells exposed to 8 kHz, 80dB sound 8kHz_80_dB_data_table.png Data Table For Stationary Phase Cells vs Cells exposed to 8 kHz, 80dB sound 8_kHz_100_dB_sound.png Stationary Phase Cells vs Cells exposed to 8 kHz, 100dB sound 8_kHz_100_dB_sound_data_table.png Data Table for Stationary Phase Cells vs Cells exposed to 8 kHz, 100dB sound 2kHz_100_dB_sound.png Stationary Phase Cells vs Cells exposed to 2 kHz, 100dB sound 2_kHz_100_dB_data_table.png Data Table for Stationary Phase Cells vs Cells exposed to 2 kHz, 100dB sound 2_kHz_100_dB_comparative_graph.png This Graph describes the expression levels of incubated cells divided by the expression of the cells in stationary phase cells and their response to sound. 2_kHz_100_dB_comparative_graph_data_table.png Data table for expression levels of incubated cells divided by the expression of the cells in stationary phase cells and their response to sound.

User Reviews

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