Difference between revisions of "Part:BBa K2589002"
 
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Our protein induction and purification results resulted in very faint bands that may have corresponded to our protein, but were too faint to make any real or definitive claims regarding its validity.  
 
Our protein induction and purification results resulted in very faint bands that may have corresponded to our protein, but were too faint to make any real or definitive claims regarding its validity.  
  
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                        <img alt="Lomassie Gel" id="img" src="https://static.igem.org/mediawiki/2018/d/d1/T--WLC-Milwaukee--CTERMHISCoomassie.png">
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<br> <br> <br>https://static.igem.org/mediawiki/2018/d/d1/T--WLC-Milwaukee--CTERMHISCoomassie.png <br> <br> <br><br> <br> <br>
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<br> The X-short J-protein from Lambda phage with a C-terminal His-tag was expressed with the pTrc99A promoter and a purification attempt was made using a Qiagen Ni-NTA spin kit. Samples were run on a 10% PAGE gel and stained with Coomassie blue stain. The red box is around a band that may correspond to the target purified protein. The J-protein fragment should be approximately 28 kD.<br>
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                        <p>The X-short J-protein from Lambda phage with a C-terminal His-tag was expressed with the pTrc99A promoter and a purification attempt was made using a Qiagen Ni-NTA spin kit. Samples were run on a 10% PAGE gel and stained with Coomassie blue stain. The red box is around a band that may correspond to the target purified protein. The J-protein fragment should be approximately 28 kD.</p>  </div>
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Latest revision as of 03:55, 18 October 2018


X-short J-Protein HIS (trc-promoter)

This part is a composite of the 2018 WLC-iGEM lambda phage X-Short J-Protein with C-Terminal His tag (BBa_K2589000) downstream of the Ptac-promoter region of BBa_K180000 allowing for Isopropyl β-D-1-thiogalactopyranoside (IPTG) induction of the coding region.


Our protein induction and purification results resulted in very faint bands that may have corresponded to our protein, but were too faint to make any real or definitive claims regarding its validity.







T--WLC-Milwaukee--CTERMHISCoomassie.png






The X-short J-protein from Lambda phage with a C-terminal His-tag was expressed with the pTrc99A promoter and a purification attempt was made using a Qiagen Ni-NTA spin kit. Samples were run on a 10% PAGE gel and stained with Coomassie blue stain. The red box is around a band that may correspond to the target purified protein. The J-protein fragment should be approximately 28 kD.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]