Difference between revisions of "Part:BBa K2549060"
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It is another version of minimal CMV which provides very low basal expression and high maximal expression after induction. Minimal CMV2 shows a better sensitivity of responsing to transcription regulation<ref>Loew R, Heinz N, Hampf M, Bujard H, Gossen M. Improved Tet-responsive promoters with minimized background expression. BMC Biotechnology. 2010;10:81. doi:10.1186/1472-6750-10-81.</ref>. | It is another version of minimal CMV which provides very low basal expression and high maximal expression after induction. Minimal CMV2 shows a better sensitivity of responsing to transcription regulation<ref>Loew R, Heinz N, Hampf M, Bujard H, Gossen M. Improved Tet-responsive promoters with minimized background expression. BMC Biotechnology. 2010;10:81. doi:10.1186/1472-6750-10-81.</ref>. | ||
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===Biology=== | ===Biology=== | ||
| − | [[File:Transient transfection analysis of the new Ptet promoters in HeLa-EM2 cells.png|240px|thumb|Loew R. et al stated ''Using both plasmid-based and retroviral gene delivery, our analysis revealed that especially background expression levels could be significantly reduced when compared to previously established "standard" promoter designs. Our results also demonstrate the possibility to fine-tune expression levels in non-clonal cell populations. They also imply differences regarding the requirements for tight regulation and high level induction between transient and stable gene transfer systems.'']] | + | [[File:Transient transfection analysis of the new Ptet promoters in HeLa-EM2 cells.png|none|240px|thumb|Loew R. et al stated ''Using both plasmid-based and retroviral gene delivery, our analysis revealed that especially background expression levels could be significantly reduced when compared to previously established "standard" promoter designs. Our results also demonstrate the possibility to fine-tune expression levels in non-clonal cell populations. They also imply differences regarding the requirements for tight regulation and high level induction between transient and stable gene transfer systems.'']] |
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Latest revision as of 03:55, 18 October 2018
minimal CMV2
It is another version of minimal CMV which provides very low basal expression and high maximal expression after induction. Minimal CMV2 shows a better sensitivity of responsing to transcription regulation[1].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Biology
Loew R. et al stated Using both plasmid-based and retroviral gene delivery, our analysis revealed that especially background expression levels could be significantly reduced when compared to previously established "standard" promoter designs. Our results also demonstrate the possibility to fine-tune expression levels in non-clonal cell populations. They also imply differences regarding the requirements for tight regulation and high level induction between transient and stable gene transfer systems.
References
- ↑ Loew R, Heinz N, Hampf M, Bujard H, Gossen M. Improved Tet-responsive promoters with minimized background expression. BMC Biotechnology. 2010;10:81. doi:10.1186/1472-6750-10-81.