Difference between revisions of "Part:BBa K2876015"
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<partinfo>BBa_K2876015 short</partinfo>
 
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This sequence produces a single-chain antibody that targets the immunoprotein interleukin B1 (BBa_K2876014) (https://parts.igem.org/Part:BBa_K2876014). We fused tthis antibody  to the alpha subunit of RNA-polymerase III (BBa_K2876002) (https://parts.igem.org/Part:BBa_K2876002) described in Dove, Joung, and Hochschild in order to a fusion protein capable of activating transcription in the presence of IL1B and another antibody fusion protein. For our reporter we used the previously described pOL2-62 from Dove, Joung, and Hochschild fused to a RFP (BBa_K2876000) (https://parts.igem.org/Part:BBa_K2876000).
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This sequence produces a single-chain antibody that targets the immunoprotein interleukin B1 (BBa_K2876014) (https://parts.igem.org/Part:BBa_K2876014). We fused this antibody  to the alpha subunit of RNA-polymerase III (BBa_K2876002) (https://parts.igem.org/Part:BBa_K2876002) described in Dove, Joung, and Hochschild in order to a fusion protein capable of activating transcription in the presence of IL1B and another antibody fusion protein. For our reporter we used the previously described pOL2-62 from Dove, Joung, and Hochschild fused to a RFP (BBa_K2876000) (https://parts.igem.org/Part:BBa_K2876000).
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2876015 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2876015 </partinfo>
  
  
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<partinfo>BBa_K2876015 parameters</partinfo>
 
<partinfo>BBa_K2876015 parameters</partinfo>
 
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==MIT_MAHE 2020==
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'''Summary'''
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Interleukin-1β (IL-1β), belongs to the interleukin-1 (IL-1) cytokine family. It is a pro-inflammatory cytokine and key in mediating body's responses to microbial infection, immunological reactions, and tissue injury. Processing of IL-1β precursor to active form can be done using various proteases. It is involved in mediating inflammation, initiating or increasing a wide variety of non-structural, function associated genes that are usually expressed during inflammation. There are now full cDNA sequences and genomic organisation of IL-1 beta taken from bird, amphibian, bony fish and cartilaginous fish, with many of these genes having been obtained using an homology cloning approach . This part produces a single-chain antibody that targets the immunoprotein interleukin B1.
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==References==
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1. Husain, M., Bird, S., van Zwieten, R., Secombes, C. J., & Wang, T. (2012). Cloning of the IL-1β3 gene and IL-1β4 pseudogene in salmonids uncovers a second type of IL-1β gene in teleost fish. Developmental and comparative immunology, 38(3), 431–446. https://doi.org/10.1016/j.dci.2012.07.010
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                                                                                                                                                                                      2. Bird, S., Zou, J., Wang, T., Munday, B., Cunningham, C., & Secombes, C. J. (2002). Evolution of interleukin-1beta. Cytokine & growth factor reviews, 13(6), 483–502. https://doi.org/10.1016/s1359-6101(02)00028-x

Latest revision as of 18:33, 23 October 2020


anti-IL1B scFv1

This sequence produces a single-chain antibody that targets the immunoprotein interleukin B1 (BBa_K2876014) (https://parts.igem.org/Part:BBa_K2876014). We fused this antibody to the alpha subunit of RNA-polymerase III (BBa_K2876002) (https://parts.igem.org/Part:BBa_K2876002) described in Dove, Joung, and Hochschild in order to a fusion protein capable of activating transcription in the presence of IL1B and another antibody fusion protein. For our reporter we used the previously described pOL2-62 from Dove, Joung, and Hochschild fused to a RFP (BBa_K2876000) (https://parts.igem.org/Part:BBa_K2876000).

Sequence and Features BBa_K2876015


MIT_MAHE 2020

Summary

Interleukin-1β (IL-1β), belongs to the interleukin-1 (IL-1) cytokine family. It is a pro-inflammatory cytokine and key in mediating body's responses to microbial infection, immunological reactions, and tissue injury. Processing of IL-1β precursor to active form can be done using various proteases. It is involved in mediating inflammation, initiating or increasing a wide variety of non-structural, function associated genes that are usually expressed during inflammation. There are now full cDNA sequences and genomic organisation of IL-1 beta taken from bird, amphibian, bony fish and cartilaginous fish, with many of these genes having been obtained using an homology cloning approach . This part produces a single-chain antibody that targets the immunoprotein interleukin B1.

References

1. Husain, M., Bird, S., van Zwieten, R., Secombes, C. J., & Wang, T. (2012). Cloning of the IL-1β3 gene and IL-1β4 pseudogene in salmonids uncovers a second type of IL-1β gene in teleost fish. Developmental and comparative immunology, 38(3), 431–446. https://doi.org/10.1016/j.dci.2012.07.010 

                                                                                                                                                                                      2. Bird, S., Zou, J., Wang, T., Munday, B., Cunningham, C., & Secombes, C. J. (2002). Evolution of interleukin-1beta. Cytokine & growth factor reviews, 13(6), 483–502. https://doi.org/10.1016/s1359-6101(02)00028-x