Difference between revisions of "Part:BBa K2588024"
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| − | <p>This part consists of the genes <a href="https://parts.igem.org/Part:BBa_K2588015">LeuABCD</a>, <a href="https://parts.igem.org/Part:BBa_K2588016">kivD</a> and <a href="https://parts.igem.org/Part:BBa_K2588017">ADH2</a>. It produces 3- | + | <p>This part consists of the genes <a href="https://parts.igem.org/Part:BBa_K2588015">LeuABCD</a>, <a href="https://parts.igem.org/Part:BBa_K2588016">kivD</a> and <a href="https://parts.igem.org/Part:BBa_K2588017">ADH2</a>. It produces 3-methyl-1-butanol according to the pathway seen in fig. 1.<sup>1</sup></p> |
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Latest revision as of 02:35, 18 October 2018
3-methyl-1-butanol synthesis module, glucose-induced
This part consists of the genes LeuABCD, kivD and ADH2. It produces 3-methyl-1-butanol according to the pathway seen in fig. 1.1
Fig 1: Metabolic pathway for amino acid biosynthesis and 3-methyl-1-butanol production. All genes are from E. coli unless otherwise noted. BS B. subtilis, LL L. lactis, SC S. cerevisiae.1
References
- Connor, M. R., Cann, A. F. & Liao, J. C. 3-Methyl-1-butanol production in Escherichia coli: random mutagenesis and two-phase fermentation. Appl. Microbiol. Biotechnol. 86, 1155–1164 (2010).
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 144
Illegal NheI site found at 167 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 7747
Illegal XhoI site found at 570 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1328
Illegal AgeI site found at 6485 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 7573
Illegal SapI.rc site found at 4405
Sequence and Features
Assembly Compatibility: