Difference between revisions of "Part:BBa K2779050"
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hemABCDEF | hemABCDEF | ||
| − | [[File:T--UAlberta--PPIXPathway.png| | + | Our full construct APIS encodes for the genes HemA- HemF, which are the enzymes required for the production of protoporphyrin IX, a tetrapyrrole compound and is an intermediate in the heme biosynthesis pathway. The composite was assembled using 4 gblocks, and have HemB, HemE and HemF coded on the complementary strand, with T7 promoters, BBa_B0034 RBS and bidirectional terminators. |
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| + | [[File:T--UAlberta--PPIXPathway.png|900px]] | ||
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Latest revision as of 01:16, 18 October 2018
APIS Device
hemABCDEF
Our full construct APIS encodes for the genes HemA- HemF, which are the enzymes required for the production of protoporphyrin IX, a tetrapyrrole compound and is an intermediate in the heme biosynthesis pathway. The composite was assembled using 4 gblocks, and have HemB, HemE and HemF coded on the complementary strand, with T7 promoters, BBa_B0034 RBS and bidirectional terminators.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1249
Illegal NheI site found at 2422 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1264
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1713
Illegal NgoMIV site found at 4974
Illegal NgoMIV site found at 5364 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 4164