Difference between revisions of "Part:BBa K2588015"
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<h2>Usage and Biology</h2> | <h2>Usage and Biology</h2> | ||
| − | <p>leuABCD is a gene naturally occurring in the genome of <i>E. coli.</i> Its translation leads to the formation of four different proteins which are involved in the synthesis pathway of L-Leucine. LeuA converts 2-ketoisovalerate into 2-isopropylmalate. LeuB and LeuC then convert it into 3-isopropylmalate. LeuD converts it into 2-isopropyl-3-oxosuccinate and 2-isopropyl-3-oxosuccinate then spontaneously transforms into 2-ketoisocaproate,<sup>1<sup> which is the substrate for the following pathway to form 3-Methyl-1-butanole.<sup>2<sup></p | + | <p>leuABCD is a gene naturally occurring in the genome of <i>E. coli.</i> Its translation leads to the formation of four different proteins which are involved in the synthesis pathway of L-Leucine. LeuA converts 2-ketoisovalerate into 2-isopropylmalate. LeuB and LeuC then convert it into 3-isopropylmalate. LeuD converts it into 2-isopropyl-3-oxosuccinate and 2-isopropyl-3-oxosuccinate then spontaneously transforms into 2-ketoisocaproate,<sup>1</sup> which is the substrate for the following pathway to form 3-Methyl-1-butanole.<sup>2</sup></p> |
<p>LeuABCD is part of the 3MB module, <a href="https://parts.igem.org/Part:BBa_K2588024">BBa_K2588024</a></p> | <p>LeuABCD is part of the 3MB module, <a href="https://parts.igem.org/Part:BBa_K2588024">BBa_K2588024</a></p> | ||
Latest revision as of 00:42, 18 October 2018
Leu Operon coding sequences from E. coli
We designed leuABCD to make E. coli produce 3-Methyl-1-butanole as a lure molecule to attract mosquitos. leuABCD is only one part of this lure module. leuABCD consists of four genes leuA, leuB, leuC and leuD. Its function is to catalyze the reaction of 2-ketoisovalerate to 2-ketoisocaproate.
Usage and Biology
leuABCD is a gene naturally occurring in the genome of E. coli. Its translation leads to the formation of four different proteins which are involved in the synthesis pathway of L-Leucine. LeuA converts 2-ketoisovalerate into 2-isopropylmalate. LeuB and LeuC then convert it into 3-isopropylmalate. LeuD converts it into 2-isopropyl-3-oxosuccinate and 2-isopropyl-3-oxosuccinate then spontaneously transforms into 2-ketoisocaproate,1 which is the substrate for the following pathway to form 3-Methyl-1-butanole.2
LeuABCD is part of the 3MB module, BBa_K2588024
References
- Connor, M.R., Cann, A.F. & Liao, J.C. Appl Microbiol Biotechnol (2010) 86: 1155.
- Engineering of an Escherichia coli Strain for the Production of 3-Methyl-1-Butanol, Michael R. Connor, James C. Liao, Appl. Environ. Microbiol. Sep 2008
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4514
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 3252
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 4340
Illegal SapI.rc site found at 1172