Difference between revisions of "Part:BBa K2560302"

Latest revision as of 23:54, 17 October 2018

Malonyl-CoA Sensor. This part is a PhytoBrick variant

piGEM2502_fapO

This part contains the fapO sequence, as well as a strong artificial promoter P_23100. FapO is the binding site for the repressor protein FapR. When FapR is present, transcription of the coding sequence controlled by J_23100 is blocked. When FapR binds Malonyl-CoA it leaves the fapO site and transcription can occur.

Usage and Biology

This part acts as a promoter controlled by the repressor FapR.
The system was taken from B. subtilis 168
Malonyl-CoA is a normal metabolite and completely harmless. For induction, a concentration between 2 and 25 mM can be used.
We designed this biobrick in order to get a system with a usable readout for the intracellular Malonyl-CoA concentration.
Can with be combined with a several reporter genes. We had the best results using the LUX operon.

File:T--Marburg--SensorConstructMalonylCoA-01.png

Figure 1
This is a schematic, illustrating the genetic context and assembly of the Malonyl-CoA sensor

' Figure 1
This is a schematic, illustrating the working mechanism of the Malonyl-CoA sensor

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 17: / Line 17: @@
 <li>Can with be combined with a several reporter genes. We had the best results using the LUX operon.</li>
 </ul>
+<div style="width:100%;display:flex;flex-direction:row;flex-wrap: wrap; justify-content:space-evenly; align-items:center;">
+[[File:T--Marburg--SensorConstructMalonylCoA-01.png|600px|thumb|left| <b> Figure 1 </b> <br>This is a schematic, illustrating the genetic context and assembly of the Malonyl-CoA sensor]]
+</div>