Difference between revisions of "Part:BBa S04038:Design"
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<partinfo>BBa_S04038 short</partinfo> | <partinfo>BBa_S04038 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
+ | This is the gene pabB + b0034 RBS placed in front of the b0015 terminator. Eventually this part will be ligated with pabA + the j23100 promoter to create a plasmid containing the two necessary genes of the para-aminobenzoic acid synthesis pathway. | ||
− | + | This plasmid can then be overexpressed to test the effects of increasing pABA production on total folate concentrations. | |
===Source=== | ===Source=== | ||
− | + | pabB was extracted from the genome of L.lactis subspe. IL1403. | |
===References=== | ===References=== |
Latest revision as of 03:51, 29 July 2008
S03977:B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is the gene pabB + b0034 RBS placed in front of the b0015 terminator. Eventually this part will be ligated with pabA + the j23100 promoter to create a plasmid containing the two necessary genes of the para-aminobenzoic acid synthesis pathway.
This plasmid can then be overexpressed to test the effects of increasing pABA production on total folate concentrations.
Source
pabB was extracted from the genome of L.lactis subspe. IL1403.