Difference between revisions of "Part:BBa K2765021:Design"
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To determine whether overexpression of <i>yno1</i> can induce the endogenous ROS accumulation in yeast, we constructed an expression plasmid based on pESC-Leu, in which the cloned <i>yno1</i> is driven by GAL1 promoter, and thus the target gene is induced by galactose and repressed by glucose. | To determine whether overexpression of <i>yno1</i> can induce the endogenous ROS accumulation in yeast, we constructed an expression plasmid based on pESC-Leu, in which the cloned <i>yno1</i> is driven by GAL1 promoter, and thus the target gene is induced by galactose and repressed by glucose. | ||
| − | [[Image: T--BIT-China--iGEM2018-Partsregulator-4.png |center| | + | [[Image: T--BIT-China--iGEM2018-Partsregulator-4.png |center|300px|]] |
| − | + | Obtained gene <i>yno1</i> though clone from yeast genome. | |
| − | + | ||
| − | [[Image: T--BIT-China--iGEM2018-Partsregulator-6.png |center| | + | [[Image: T--BIT-China--iGEM2018-Partsregulator-5.png |center|600px|]] |
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| + | we constructed an expression plasmid based on pESC-Leu, ligase gene <i>yno1</i> into it, We use <i>E.coli</i> as our host cell to construct the | ||
| + | rcombinant plasmid, then transformed it into <i>Saccharomyces cerevisiae</i>. | ||
| + | |||
| + | [[Image: T--BIT-China--iGEM2018-Partsregulator-6.png |center|600px|]] | ||
| + | |||
| + | Here shows the result of Colony PCR in <i>E.coli</i>. | ||
Latest revision as of 23:43, 17 October 2018
To determine whether overexpression of yno1 can induce the endogenous ROS accumulation in yeast, we constructed an expression plasmid based on pESC-Leu, in which the cloned yno1 is driven by GAL1 promoter, and thus the target gene is induced by galactose and repressed by glucose.
Obtained gene yno1 though clone from yeast genome.
we constructed an expression plasmid based on pESC-Leu, ligase gene yno1 into it, We use E.coli as our host cell to construct the rcombinant plasmid, then transformed it into Saccharomyces cerevisiae.
Here shows the result of Colony PCR in E.coli.