Difference between revisions of "Part:BBa K2725016"
 
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Serine amber suppressor tRNA gene (supD) downstream of J23108 Anderson promoter. When co-transformed, this part allows the expression of a protein coding gene with up to 5 serine codons substituted for amber stop codons (TAG) - such as BBa_K2725012 - P1003 Kanamycin resistance gene with 5 amber substitutions.
 
Serine amber suppressor tRNA gene (supD) downstream of J23108 Anderson promoter. When co-transformed, this part allows the expression of a protein coding gene with up to 5 serine codons substituted for amber stop codons (TAG) - such as BBa_K2725012 - P1003 Kanamycin resistance gene with 5 amber substitutions.
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https://static.igem.org/mediawiki/2018/e/ec/T--Edinburgh_UG--400.png
 
https://static.igem.org/mediawiki/2018/e/ec/T--Edinburgh_UG--400.png
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Figure 1. Growth curves of double transformed Top10 E. coli strains in 400 μg/mL Kanamycin.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 22:30, 17 October 2018


J23108 - supD - B1006

Serine amber suppressor tRNA gene (supD) downstream of J23108 Anderson promoter. When co-transformed, this part allows the expression of a protein coding gene with up to 5 serine codons substituted for amber stop codons (TAG) - such as BBa_K2725012 - P1003 Kanamycin resistance gene with 5 amber substitutions.


T--Edinburgh_UG--400.png

Figure 1. Growth curves of double transformed Top10 E. coli strains in 400 μg/mL Kanamycin.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 77
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 80