Difference between revisions of "Part:BBa K2624002:Experience"

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===Applications of BBa_K2624002===
 
===Applications of BBa_K2624002===
We applied indirect immunofluorescence to prove the expression (Figure2 A) and nuclear translocation (Figure2 B) of NS5B-NLS.
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To verify that the improved component is valid, we constructed two plasmids. One is NLS+(containing this part), and another is NLS- (containing BBa_K1442028).We applied indirect immunofluorescence to prove the expression (Figure.A) and nuclear translocation (Figure.B) of these parts.
  
 
https://static.igem.org/mediawiki/2018/5/56/T--CPU_CHINA--hp-qidongzi.png
 
https://static.igem.org/mediawiki/2018/5/56/T--CPU_CHINA--hp-qidongzi.png
  
Figure.The expression and nuclear translocation of NS5BNLS
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Figure.The expression and nuclear translocation of NLS+ and NLS-.
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===User Reviews===
 
===User Reviews===
 
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Latest revision as of 03:32, 8 December 2018


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2624002

To verify that the improved component is valid, we constructed two plasmids. One is NLS+(containing this part), and another is NLS- (containing BBa_K1442028).We applied indirect immunofluorescence to prove the expression (Figure.A) and nuclear translocation (Figure.B) of these parts.

T--CPU_CHINA--hp-qidongzi.png

Figure.The expression and nuclear translocation of NLS+ and NLS-.

User Reviews

UNIQ7b744c95cd0e450c-partinfo-00000000-QINU UNIQ7b744c95cd0e450c-partinfo-00000001-QINU