Difference between revisions of "Part:BBa K2685007"

Latest revision as of 20:49, 17 October 2018

Detecting parts

This part is our part of the test to detect tetracycline in the environment. Because E. coli is not tolerant to tetracycline, we introduced a resistance mechanism to expand its tolerance to tetracycline, which can be detected at a wider concentration of tetracycline. The ABS value of E. coli was determined by comparing with that of E.coli. The results showed that the highest concentration of tetracycline was 10μg/ml in E.coli and 110μg /ml in E.coli.

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The following picture shows the contents of NKU_China’s and our collaboration. Repeated two experiments at 0.2% inoculation level, the MIC value of detection parts can also be measured to show that detection parts are tolerable.

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Moreover, the tetracycline concentration detected by the detection parts can be as low as 0.005 μg/ml, the detection performance is more stable and the detection accuracy is higher.

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Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1768
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1622
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1068
Illegal NgoMIV site found at 1228
Illegal NgoMIV site found at 1596
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 69

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2685007 short</partinfo>
-<p>This part is our part of the test to detect tetracycline in the environment. Because E. coli is not tolerant to tetracycline, </p>
+<p>This part is our part of the test to detect tetracycline in the environment. Because <i>E. coli</i> is not tolerant to tetracycline, we introduced a resistance mechanism to expand its tolerance to tetracycline, which can be detected at a wider concentration of tetracycline. The ABS value of <i>E. coli</i> was determined by comparing with that of <i>E.coli</i>. The results showed that the highest concentration of tetracycline was 10μg/ml in<i> E.coli</i> and 110μg /ml in <i>E.coli</i>.</p>
-<p>we introduced a resistance mechanism to expand its tolerance to tetracycline, which can be detected at a wider concentration </p>
-<p>of tetracycline. The ABS value of E. coli was determined by comparing with that of E.coli. The results showed that the highest</p> <p>concentration of tetracycline was 10μg/ml in E.coli and 110μg /ml in E.coli.</p>
    <div class="at-user__avatar" align="center">
 "https://static.igem.org/mediawiki/parts/e/e6/T--TUST_China--part_document_072.png"
                      </div>
-<p>The following picture shows the contents of NKU_China’s and our collaboration. Repeated two experiments at 0.2% inoculation </p>
+<p>The following picture shows the contents of NKU_China’s and our collaboration. Repeated two experiments at 0.2% inoculation level, the MIC value of detection parts can also be measured to show that detection parts are tolerable.</p>
-<p>level, the MIC value of detection parts can also be measured to show that detection parts are tolerable.</p>
 <div class="at-user__avatar" align="center">
 "https://static.igem.org/mediawiki/parts/7/7c/T--TUST_China--MIC.png"
                      </div>
-<p>Moreover, the tetracycline concentration detected by the detection parts can be as low as 0.005 μg/ml, the detection performance</p>
+<p>Moreover, the tetracycline concentration detected by the detection parts can be as low as 0.005 μg/ml, the detection performance is more stable and the detection accuracy is higher.</p>
-<p> is more stable and the detection accuracy is higher.</p>
   <div class="at-user__avatar" align="center">
 "https://static.igem.org/mediawiki/parts/c/c9/T--TUST_China--4.png"