Difference between revisions of "Part:BBa K2719006:Experience"

Latest revision as of 22:19, 17 October 2018

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Please enter how you used this part and how it worked out.

Applications of BBa_K2719006

This part was cloned on pSB1C3 using EcoRI and PstI and latter transformed into E.coli DH5a. The colonies obtained can be seen on figure 1.

Figure 1. Colonies transformed with Collagen V-like.

Then the plasmid was extracted and run by duplicated on an agarose gel electrophoresis (Figure 2)

Figure 2. 0.85% agarose gel with GelRed: MW) 1Kb plus from NEB; 3) Collagen V- like (BBa_K2719006) V'; 4) Collagen V- like (BBa_K2719006) V

Latter it was digested using NotI to probe the presence of the insert, the restriction was also run on a agarose gel electrophoresis.

Figure 3. 0.85% agarose gel with GelRed: MW) 2-Log DNA Ladder; 2) Collagen V- like (BBa_K2719006) restricted with NotI

User Reviews

UNIQd6c663c2b25ccb0f-partinfo-00000000-QINU UNIQd6c663c2b25ccb0f-partinfo-00000001-QINU

@@ Line 13: / Line 13: @@
 <p>Then the plasmid was extracted and run by duplicated on an agarose gel electrophoresis (Figure 2)</p>
 [[file:T--TecCEM--Col5Extraction.png|500px]]
-<p><i>Figure 2.</i> 0.85% agarose gel with GelRed: MW) 1Kb plus from NEB; 3) Collagen V- like (K2719006) V'; 4) Collagen V- like (K2719006) V''</p>
+<p><i>Figure 2.</i> 0.85% agarose gel with GelRed: MW) 1Kb plus from NEB; 3) Collagen V- like (BBa_K2719006) V'; 4) Collagen V- like (BBa_K2719006) V''</p>
 <p>Latter it was digested using NotI to probe the presence of the insert, the restriction was also run on a agarose gel electrophoresis.</p>
+[[file:T--TecCEM--ResK2719006.png|500px]]
+<p><i>Figure 3.</i> 0.85% agarose gel with GelRed: MW) 2-Log DNA Ladder; 2) Collagen V- like (BBa_K2719006) restricted with NotI</p>
 ===User Reviews===