Difference between revisions of "Part:BBa K2717023"
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In this composited part, we link GFP with upstream CI to control the whole coding. We design this part to test the efficiency of growth activation of CI protein. And we first use the sequence in part with the promoter from BBa_K1139150. | In this composited part, we link GFP with upstream CI to control the whole coding. We design this part to test the efficiency of growth activation of CI protein. And we first use the sequence in part with the promoter from BBa_K1139150. | ||
| + | function:We design this part to test the efficiency of growth activation of CI protein. The improvement of green fluorescence can be detected under the induction of IPTG. Comparing with control, CI protein has the ability to improve the expression of downstream protein by 150%. | ||
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| + | https://static.igem.org/mediawiki/2018/6/65/T--BNU-China--_R18.png | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 04:00, 18 October 2018
λcI-xiao(λ repressor-lac operator-GFP)
In this composited part, we link GFP with upstream CI to control the whole coding. We design this part to test the efficiency of growth activation of CI protein. And we first use the sequence in part with the promoter from BBa_K1139150.
function:We design this part to test the efficiency of growth activation of CI protein. The improvement of green fluorescence can be detected under the induction of IPTG. Comparing with control, CI protein has the ability to improve the expression of downstream protein by 150%.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 766
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 742
Illegal BsaI.rc site found at 1540