Difference between revisions of "Part:BBa K2569031"
(→‎Nicotine Degradation Pathway)
 
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Expressing this BioBrick in <i>E. coli</i> cells enables the bacterium to degrade nicotine. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in <i>E. coli</i> can convert nicotine to pseudo-oxidation can be achieved by resting cells of E. coli as a catalyst in a whole-cell reaction.
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Expressing this BioBrick in <i>Pseudomonas fluorescence</i>-pf5 cells enables the bacterium to degrade nicotine. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in <i>P.fluorescence</i>-pf5 can convert nicotine to pseudo-oxidation can be achieved by resting cells of <i>P.fluorescence</i>-pf5 as a catalyst in a whole-cell reaction.So, with our strong promoper PdnaA enhancing, NicA2 is overexpressed and survived in high concentration of nicotin. And nicotin can get fully degradated.
  
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===Nicotine Degradation Pathway===
 
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<img src="https://static.igem.org/mediawiki/2018/7/70/T--SKLMT-China--nicotine-pathway.png" alt="Nicotine degradation pathway" />
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  https://static.igem.org/mediawiki/2018/7/70/T--SKLMT-China--nicotine-pathway.png
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  Fig. Nicotin degradation pathway.
 
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===Usage and Biology===
 
===Usage and Biology===
  

Latest revision as of 19:02, 17 October 2018


PdnaA -NicA2

Expressing this BioBrick in Pseudomonas fluorescence-pf5 cells enables the bacterium to degrade nicotine. NicA2 is a monomer with a size of 53 kDa t, catalyzing the key first step in nicotine catalytic metabolism. Heterologously overexpressing NicA2 in P.fluorescence-pf5 can convert nicotine to pseudo-oxidation can be achieved by resting cells of P.fluorescence-pf5 as a catalyst in a whole-cell reaction.So, with our strong promoper PdnaA enhancing, NicA2 is overexpressed and survived in high concentration of nicotin. And nicotin can get fully degradated.

Nicotine Degradation Pathway

  T--SKLMT-China--nicotine-pathway.png
  Fig. Nicotin degradation pathway.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1393
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]