Difference between revisions of "Part:BBa K390501:Experience"

(→‎User Reviews)
(→‎User Reviews)
 
(2 intermediate revisions by the same user not shown)
Line 27: Line 27:
 
<I>Username</I>
 
<I>Username</I>
 
|width='60%' valign='top'|
 
|width='60%' valign='top'|
−
Enter the review inofrmation here.
 
−
Available Phasin biobrick (BBa_K208001) are found in Lac promoter-Phasin-HlyA biobrick, BBa_K390501. Through sequencing, it was found to contain a stop codon. A set of PCR primers was designed and used to remove the stop codon. In the primer design, a HindIII restriction enzyme site, which is not present in the original biobrick, was introduced and allow the confirmation of the success stop codon removal.
 
  
−
https://static.igem.org/mediawiki/parts/b/b0/T--Edinburgh_OG_BBa_K2739007--image_5.jpeg
+
Available Phasin biobrick (BBa_K208001) found in Lac promoter-Phasin-HlyA biobrick, BBa_K390501. Through sequencing, it was found to contain a stop codon. A set of PCR primers was designed and used to remove the stop codon. In the primer design, a HindIII restriction enzyme site, which is not present in the original biobrick, was introduced and allow the confirmation of the success stop codon removal.
 +
 
 +
https://static.igem.org/mediawiki/parts/4/42/T--Edinburgh_OG_BBa_K2739007--image_6.jpeg
 
Figure 1. The sequence alignment of BBa_K390501, LacP-Phasin-Hly-VR-170918-08-27(The sequencing result of BBa K2739007) and original-phasinHlyA-VR-170918-08-28 (The sequencing result of BBa_K390501) via Benchling. The selected (blue) part of the sequence indicated the stop codon.  
 
Figure 1. The sequence alignment of BBa_K390501, LacP-Phasin-Hly-VR-170918-08-27(The sequencing result of BBa K2739007) and original-phasinHlyA-VR-170918-08-28 (The sequencing result of BBa_K390501) via Benchling. The selected (blue) part of the sequence indicated the stop codon.  
 
|};
 
|};
 
<!-- End of the user review template -->
 
<!-- End of the user review template -->
 
<!-- DON'T DELETE --><partinfo>BBa_K390501 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K390501 EndReviews</partinfo>

Latest revision as of 18:22, 17 October 2018

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K390501

This part is used for phasin secretion. The same general structure of this composite part could be used to for the secretion of other proteins by substituting phasin for another part of interest. These would need to be made Silver-fusion compatible.

User Reviews

UNIQ6c9c9adeea52000f-partinfo-00000000-QINU UNIQ6c9c9adeea52000f-partinfo-00000001-QINU

No review score entered. Username

Available Phasin biobrick (BBa_K208001) found in Lac promoter-Phasin-HlyA biobrick, BBa_K390501. Through sequencing, it was found to contain a stop codon. A set of PCR primers was designed and used to remove the stop codon. In the primer design, a HindIII restriction enzyme site, which is not present in the original biobrick, was introduced and allow the confirmation of the success stop codon removal.

T--Edinburgh_OG_BBa_K2739007--image_6.jpeg Figure 1. The sequence alignment of BBa_K390501, LacP-Phasin-Hly-VR-170918-08-27(The sequencing result of BBa K2739007) and original-phasinHlyA-VR-170918-08-28 (The sequencing result of BBa_K390501) via Benchling. The selected (blue) part of the sequence indicated the stop codon.

;

UNIQ6c9c9adeea52000f-partinfo-00000003-QINU