Difference between revisions of "Part:BBa K2557002"
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Latest revision as of 18:28, 17 October 2018
Bxb1 attB-RFP-Bxb1 attP
We added Bxb1 recombination sites, attB and attP, at both ends of the reporter gene RFP (Inverted). When Bxb1 is expressed, attB and attP are recognized, and the sequence of RFP is inverted to enable normal expression.
Usage and Biology
Sequence and Features
Characterization
Fig. 1 Fluorescence microscope observation of HEK 293T undergone different experimental treatments. Transfection of different amounts of plasmids containing recombinase genes into cells.
Fig. 1 shows that this composite part works fine in the HEK 293T.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 837
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 16
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 36
Illegal BsaI site found at 177
Illegal BsaI.rc site found at 885
Illegal SapI site found at 794