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<h2>Overview</h2>
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<h2>Usage and Biology</h2>
 
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PhoA is a type of signal peptide linking with CotA. It can transfer CotA out of <em>E.coli</em>.  We tested the enzyme activity of our part based on the weight of our sample. Formula: laccase activity (nmol/min/g) = ΔA /ε(ABTS mmolar extinction coefficient) / d * V (total volume of reaction) / V (volume of sample in the reaction, 0.025mL) / W (sample mass, g) * V (extracted liquid added, 1mL) / T (reaction time, 3 minutes) = 130 *ΔA / W. The result is shown as following: </p>
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PhoA is a signal peptide attached to CotA coding sequence. PhoA signal peptide can help transfer CotA out of <I>E.coli</I>.   
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The enzyme activity was measured and calculated based on the weight of our sample. Formula: laccase activity (nmol/min/g) = ΔA /ε(ABTS mmolar extinction coefficient) / d * V (total volume of reaction) / V (volume of sample in the reaction, 0.025mL) / W (sample mass, g) * V (extracted liquid added, 1mL) / T (reaction time, 3 minutes) = 130 *ΔA / W.
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<p><img src="https://static.igem.org/mediawiki/2018/4/42/T--SHSBNU_China--Parts_Regis.png" style="width:50%"/></image></p>
 
<p><img src="https://static.igem.org/mediawiki/2018/4/42/T--SHSBNU_China--Parts_Regis.png" style="width:50%"/></image></p>
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For more experimental details, please visit http://2018.igem.org/Team:SHSBNU_China/Experiments
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Latest revision as of 21:09, 17 October 2018


PhoA-CotA

CotA laccase attached with PhoA signal peptide sequence


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1408
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 346


Usage and Biology

PhoA is a signal peptide attached to CotA coding sequence. PhoA signal peptide can help transfer CotA out of E.coli.

The enzyme activity was measured and calculated based on the weight of our sample. Formula: laccase activity (nmol/min/g) = ΔA /ε(ABTS mmolar extinction coefficient) / d * V (total volume of reaction) / V (volume of sample in the reaction, 0.025mL) / W (sample mass, g) * V (extracted liquid added, 1mL) / T (reaction time, 3 minutes) = 130 *ΔA / W.

For more experimental details, please visit http://2018.igem.org/Team:SHSBNU_China/Experiments